Regener8 member Orla proteins enable improved TB detection
8 Nov 2011
The UK National Physcial Laboratory (NPL) and Orla have recently completed a project demonstrating enhanced detection of interferon gamma (IFNγ), a means of diagnosing Mycobacterium tuberculosis, TB, infection.
The assay was performed on a Biacore T200 instrument using an Orla protein surface to capture anti-IFNγ antibodies and present them as a controlled oreinted surface to detect IFNγ in spiked complex culture medium containing BSA and growth factors.
Using an Orla surface allowed NPL to significantly improve the assay detect IFNγ at concentrations as low as 3nM.
A poster describing the work can viewed here.
Orla provides kits for performing such experiments on SPR, QCM and other analytical devices.
This work was funded by the Technology Strategy Board as part of the Detecting Infectiuos Disease programme.
